Inside-Out Regulation of L1 Conformation, Integrin Binding, Proteolysis, and Concomitant Cell Migration
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چکیده
منابع مشابه
Inside-Out Regulation of L1 Conformation, Integrin Binding, Proteolysis, and Concomitant Cell Migration
Previous reports on the expression of the cell adhesion molecule L1 in pancreatic ductal adenocarcinoma (PDAC) cells range from absent to high. Our data demonstrate that L1 is expressed in poorly differentiated PDAC cells in situ and that threonine-1172 (T1172) in the L1 cytoplasmic domain exhibits steady-state saturated phosphorylation in PDAC cells in vitro and in situ. In vitro studies suppo...
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Department of Pathology and Cancer Center, University of New Mexico HSC, Albuquerque, NM 87131 Department of Cell Biology and Physiology, University of New Mexico HSC, Albuquerque, NM 87131 National Flow Cytometry Resource, Los Alamos National Laboratory, Los Alamos, NM 87545
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Integrins dynamically equilibrate between three conformational states on cell surfaces. A bent conformation has a closed headpiece. Two extended conformations contain either a closed or an open headpiece. Headpiece opening involves hybrid domain swing-out and a 70 Å separation at the integrin knees, which is conveyed by allostery from the hybrid-proximal end of the βI domain to a 3 Å rearrangem...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2010
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e09-10-0900